Welcome to FIS   Sponsored By
United States
Subscribe to FIS | Register with FIS | Advertise with FIS | Newsletter | About FIS | Contact us
   


Results of this study show that the commercial microbial enhanced protein product ME-PRO® has significantly higher levels of hydrolysable protein

In vitro protein digestibility of a microbial-enhanced protein for juvenile white shrimp

Click on the flag for more information about United States UNITED STATES
Wednesday, July 01, 2020, 06:00 (GMT + 9)

MEP shows higher levels of hydrolysable protein, predicted apparent protein digestibility

Increasing demand for specialty feeds has stimulated the increase and growth of specialty ingredients that enhance the quality of feeds. The direct effects of special ingredients – such as microbial enhanced proteins (MEP; a leading alternative protein source to replace fishmeal in aquaculture diets) – can provide significant amounts of biologically active factors that can increase gut microbiota, reduce intestinal inflammation and boost metabolic processes for improved animal health. However, the optimum production of feedstuffs with optimum dry matter conversion of feed to weight depends largely on ingredient quality and nutrient availability for the species in question.

The digestibility of an ingredient refers to how much nutrition it provides, to the proportion of all its nutrients that are available to the animal to be absorbed from its intestines. It provides a measure of the nutritional value and quality of an ingredient, because highly digestible ingredients provide a higher relative amount of absorbed nutrients compared to a less digestible ingredient. The determination of digestibility of major nutrients is one of the main steps in the evaluation of their bioavailability to better fulfill the specific requirements for a given species.

View of the analytical station with titrators and central control software for pH-stat determination of protein hydrolysis at LAM-USP.

In this article, we report on a recent study we carried out to determine the in vitro protein digestibility of a commercial MEP – ME-PRO® (Prairie Aquatech, South Dakota USA) – with species-specific digestive enzymes for juvenile Pacific white shrimp (Litopenaeus vannamei).

Study setup

The digestibility study was conducted at the Aquaculture Laboratory (LAM), Oceanographic Institute, University of São Paulo (USP; São Paulo, Brazil). Samples of two soybean products were provided by Prairie Aquatech (South Dakota, USA). Samples of the soy products – non-GMO soybean meal, SBM (46.8 percent crude protein, CP) and ME-PRO® (74.6 percent CP) – had appropriate particle size (>150 microns) and were analyzed for determination of their degree of protein hydrolysis (DH, percent).

Ready-to-use frozen standardized enzyme extracts recovered from shrimp species for in vitro protein digestion assays at LAM-USP.

These samples were tested for in vitro protein digestion with standardized digestive enzymes recovered from the hepatopancreas of pond-farmed Pacific white shrimp (10 grams average weight). The hydrolysis with shrimp enzymes involved the suspension of 80 mg of ingredient protein in distilled water, with pH of the suspension set at 8.0 following the addition of the hepatopancreas enzyme extract for hydrolysis.

The pH shift and hydrolysis monitoring were automatically performed by commercial, software- controlled potentiometric titrators in temperature-controlled devices (30 ± 0.6 degrees-C). At this reaction pH (= 8.0), the enzymatic breakage of ingredient peptide bonds produces slight reduction in reaction pH that is registered and automatically neutralized by the titrator with addition of sodium hydroxide, NaOH. At the end of the reaction the amount of titrant (NaOH) expended is proportional to the number of peptide bonds cleaved and a quantitative value provided: the degree of protein hydrolysis (DH, percent).

No buffers or other chemicals were used in the analysis. If determined significant, then blank DH values were computed for the calculation of net DH of ingredient protein.(continue...)

Author: Sergio F. Nates, Ph.D. / Global Aquaculture Advocate | Read full article here

editorial@fis.com
www.fis.com


 Print


Click to know how to advertise in FIS
MORE NEWS
European Union
Jul 8, 01:30 (GMT + 9):
PRESS RELEASE: EAS events in Cork and Madeira in 2021 – an update
Russian Federation
Jul 7, 16:00 (GMT + 9):
The suburbs of Moscow will be engaged in the cultivation of Atlantic salmon
Norway
Jul 7, 15:40 (GMT + 9):
Mowi’s soy suppliers commit to 100% deforestation free supply chain
Canada
Jul 7, 15:00 (GMT + 9):
Prices │ Snow crab (Opilio) │ Newfoundland │ 2018-19-20
Australia
Jul 7, 10:00 (GMT + 9):
Other Media | SeafoodSource: Australia extends freight flight support for seafood exporters
European Union
Jul 7, 08:00 (GMT + 9):
Other Media | FiskerForum: Pelagic sector invites Greenpeace collaboration
Japan
Jul 7, 07:00 (GMT + 9):
Yanmar to Develop Hydrogen Fuel Cell System for Maritime Applications
Viet Nam
Jul 7, 03:00 (GMT + 9):
Other Media | The Fish Site: Vietnam to ramp up organic aquaculture production before 2025
Spain
Jul 7, 00:10 (GMT + 9):
23% of Spaniards have increased delivery consumption in restaurants during confinement
Norway
Jul 7, 00:10 (GMT + 9):
Press release from North Atlantic Seafood Forum, NASF
Japan
Jul 7, 00:00 (GMT + 9):
Volumes and prices │ Japanese Yellowtail (Hamachi) | Tokyo Markets | 2018-19-20
Japan
Jul 7, 00:00 (GMT + 9):
Statistics │ Import │Bottomfish from USA and Russia (2018/19/20)
United States
Jul 7, 00:00 (GMT + 9):
Total catch BSAI-GOA │ POP │week 25
United States
Jul 7, 00:00 (GMT + 9):
DAP at BSAI-GOA | Atka Mackerel, Dusky Sole, Yellowfin Sole and Pacific Cod | week 25
Norway
Jul 7, 00:00 (GMT + 9):
Other Media | fishfarmingexpert : SalMar harvests 41,000 tonnes in Q2



Lenguaje
FEATURED EVENTS
  
TOP STORIES
Bristol Bay fishermen await word on Pebble Mine permit at the height of fishing season
United States The biggest red salmon run in the world is building at Bristol Bay. Up to 50 million fish could surge into its eight river systems in coming weeks, on par with past seasons. When it’s all done,...
New knowledge about salt fish production
Norway Now a salt status has been created for the salt quality used for clipfish and salt fish production, and how the salt can affect the finished fish product. A prototype for cleaning and washing salt for...
Volumes and prices | Main fresh fish: Skipjack, sardine, mackerel, saury and squid | Tokyo Markets (May-2020)
Japan Source: FIS.com | Click to enlarge it editorial@fis.com www.fis.com...
World’s first full scale ammonia engine test - an important step towards carbon free vessels
Finland The project leaders pictured at the Sustainable Energy Catapult Centre’s test facility at Stord, Norway The technology group Wärtsilä, in close customer cooperation with Knutsen OAS Shipping AS a...
 
Maruha Nichiro Corporation
Nichirei Corporation - Headquarters
Pesquera El Golfo S.A.
Ventisqueros - Productos del Mar Ventisqueros S.A
Wärtsilä Corporation - Wartsila Group Headquarters
ITOCHU Corporation - Headquarters
BAADER - Nordischer Maschinenbau Rud. Baader GmbH+Co.KG (Head Office)
Inmarsat plc - Global Headquarters
Marks & Spencer
Tesco PLC (Supermarket) - Headquarters
Sea Harvest Corporation (PTY) Ltd. - Group Headquarters
I&J - Irvin & Johnson Holding Company (Pty) Ltd.
AquaChile S.A. - Group Headquarters
Pesquera San Jose S.A.
Nutreco N.V. - Head Office
CNFC China National Fisheries Corporation - Group Headquarters
W. van der Zwan & Zn. B.V.
SMMI - Sunderland Marine Mutual Insurance Co., Ltd. - Headquarters
Icicle Seafoods, Inc
Starkist Seafood Co. - Headquearters
Trident Seafoods Corp.
American Seafoods Group LLC - Head Office
Marel - Group Headquarters
SalMar ASA - Group Headquarters
Sajo Industries Co., Ltd
Hansung Enterprise Co.,Ltd.
BIM - Irish Sea Fisheries Board (An Bord Iascaigh Mhara)
CEFAS - Centre for Environment, Fisheries & Aquaculture Science
COPEINCA ASA - Corporacion Pesquera Inca S.A.C.
Chun Cheng Fishery Enterprise Pte Ltd.
VASEP - Vietnam Association of Seafood Exporters & Producers
Gomes da Costa
Furuno Electric Co., Ltd. (Headquarters)
NISSUI - Nippon Suisan Kaisha, Ltd. - Group Headquarters
FAO - Food and Agriculture Organization - Fisheries and Aquaculture Department (Headquarter)
Hagoromo Foods Co., Ltd.
Koden Electronics Co., Ltd. (Headquarters)
A.P. Møller - Maersk A/S - Headquarters
BVQI - Bureau Veritas Quality International (Head Office)
UPS - United Parcel Service, Inc. - Headquarters
Brim ehf (formerly HB Grandi Ltd) - Headquarters
Hamburg Süd Group - (Headquearters)
Armadora Pereira S.A. - Group Headquarters
NOAA - National Oceanic and Atmospheric Administration (Headquarters)
Mowi ASA (formerly Marine Harvest ASA) - Headquarters
Marubeni Europe Plc -UK-
Findus Ltd
Icom Inc. (Headquarter)
WWF Centroamerica
Oceana Group Limited
The David and Lucile Packard Foundation
Ajinomoto Co., Inc. - Headquarters
Friosur S.A. - Headquarters
Cargill, Incorporated - Global Headquarters
Benihana Inc.
Leardini Pescados Ltda
CJ Corporation  - Group Headquarters
Greenpeace International - The Netherlands | Headquarters
David Suzuki Foundation
Fisheries and Oceans Canada -Communications Branch-
Mitsui & Co.,Ltd - Headquarters
NOREBO Group (former Ocean Trawlers Group)
Natori Co., Ltd.
Carrefour Supermarket - Headquarters
FedEx Corporation - Headquarters
Cooke Inc. - Group Headquarters
AKBM - Aker BioMarine ASA
Seafood Choices Alliance -Headquarter-
Austevoll Seafood ASA
Walmart | Wal-Mart Stores, Inc. (Supermarket) - Headquarters
New Japan Radio Co.Ltd (JRC) -Head Office-
Gulfstream JSC
Marine Stewardship Council - MSC Worldwide Headquarters
Royal Dutch Shell plc (Headquarter)
Genki Sushi Co.,Ltd -Headquarter-
Iceland Pelagic ehf
AXA Assistance Argentina S.A.
Caterpillar Inc. - Headquarters
Tiger Brands Limited
SeaChoice
National Geographic Society
AmazonFresh, LLC - AmazonFresh

Copyright 1995 - 2020 Fish Info & Services Co.Ltd| All Rights Reserved.   DISCLAIMER